Effect of peak asymmetry on column efficiency and separation

3 Effect of peak asymmetry on column efficiency and separation 

Foley and Dorsey (1983) have considered the effect of peak tailing on column efficiency and concluded that measuring peak widths at half height (equation (2.26)) substantially overestimates the number of theoretical plates of a tailed peak. Thus resolution (equations (2.46) and (2.47)) 

Equation (2.48) was derived empirically but for symmetrical peaks ( s,o.i = 1.00), equation (2.48) and the corresponding equation obtained assuming a Gaussian distribution differ by only 0.6%  

Anon (1990) United States Pharmacopeia. National Formidary, United States Pharmacopeial Convention, Rockville, MD, pp. 1565 1566. 

Bristow, P.A. and Knox, J.H. (1977) Standardization of test conditions for high performance liquid chromatography columns, Chromatographia 10, 279 289. 

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