Effect of Acyl Donor Concentration

The IL/SC-CO2 media for 1-phenylethanol resolution, catalyzed by CALB, offers a number of advantages, such as high resolution yields, simple downstream processing, guarantying an enzyme overstabilization effect and exceptional enzyme enantioselectivity with no transformation of the not desired enantiomer, albeit apparently lower conversions were registered in this medium compared to the ones, obtained in SC-CO2. 

Kinetic resolution of racemic 1-phenylethanol was successfully performed by CALB as the enantioselective biocatalyst using vinyl acetate as the acyl donor. 

High yield, enantioselectivity and simple downstream processing make SC-CO2 and IL/SC-CO2 systems excellent media for the above menhoned kinetic resolu-hon of sec-alcohol. Pressure effects the reaction to a certain extent, which was explained by exploiting thermodynamic arguments. Besides, the yield may be improved by increasing the acyl donor concentration. 

The outlook of syntheses, based on using SC-CO2 or appropriate IL/SC-CO2 biphasic system for developing integral green chemical processes due to the physical and chemical characterishcs of these neoteric solvents and the enhanced enzyme catalytic properties seems promising. 

and Voss, E. (2001) Applied Catalysis A General, 221, 145-158. Keskin, S., Kayrak-Talay, D., Akman, U. and Horta su. 0. (2007) The Journal of Supercritical Fluids, 43 (1), 150-180. 

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