EcoRl

Econo Foam Economic evaluation EcoO EcoRl EcoRI Ecostatin... 

Fig. 4. Construction of recombinant phage in vectors derived from bacteriophage lambda where E represents the enzyme EcoRl. Other terms are defined...

The XE6 DNA was digested with several restriction enzymes and analysed via Southern blots using a probe derived fi-om the pgaW gene. A restriction map of the X.E6 clone revealed that the complete gene should be present on a 3.0 kb EcoRl fi agment (see Fig. 1). 

TABLE 7.2 Autoradiogram of the Reductive Cleavage of a 5 -32P End-Labeled 514 bp Restriction Fragment from pBR322 DNA (Ecorl/Rsal) by Cy cl open t />] indoles... 

Grable, J., Frederick, C. A., Samudzi, C., Jen-Jacobsen, L., Lesser, D., Greene, P, Boyer, H. W, Itakura, K. and Rosenberg, J. M. (1984). Two-fold symmetry of crystalhne DNA-EcoRl endonuclease recognition complexes. /. Biomol. Struct. Dynam. 1,1149-1160. 

Several unique recognition sequences in pBR322 (Pstl, EcoRl, BamHl, Sail, PvulI) are targets for different restriction endonucleases, providing sites where the plasmid can later be cut to insert foreign DNA. 

Clone by insertion at an EcoRl site in a cloning vector, (b)... 

The colicin El plasmid is a 4.43 MDa circular double stranded DNA molecule consisting of 6646 base pairs.1 Only one site is susceptible to cleavage by the restriction endonuclease ECoRl (Chapter 26) This feature has led to its widespread use in cloning of genes. 

Fig. 2. Structure of the fusion vector pSV-VNPy2bA(CH2, CH3). The VNP and y2b genes are inserted in pSVgpt between the EcoRl and BamHl sites. The BamHl site is converted to Xhol by the addition of linkers B, Bgl l H, Hin WV, P, Pvu R, EcoRl, X, Xhol, E, immunoglobulin enhancer.

Colren and Boyer reported genetic engineering technique (EcoRl enzyme). 

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