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EcoRl site specificity

When EcoRl cuts the DNA at this site, it does so in a staggered fashion. Specifically, it cuts between the G and the first A on both strands. Cutting produces two DNA fragments with the following structure ... [Pg.740]

A restriction endonuclease recognizes a set pattern of four to eight nucleotides and cleaves a DNA strand by hydrolysis of the linking phosphodiester bonds at any site that contains that particular sequence. Close to 1000 restriction endonucleases have been isolated and their specificities characterized each cleaves at specific sites, often with unique specificity. E. coli, for example, has a restriction endonuclease, EcoRl, that recognizes the hexanucleotide sequence, GAATTC, and cleaves it between G and A. [Pg.1202]

See other pages where EcoRl site specificity is mentioned: [Pg.250]    [Pg.103]    [Pg.366]    [Pg.143]    [Pg.258]    [Pg.1565]    [Pg.287]    [Pg.380]    [Pg.288]    [Pg.1998]    [Pg.156]    [Pg.431]    [Pg.1379]    [Pg.652]    [Pg.631]    [Pg.312]    [Pg.690]    [Pg.15]    [Pg.1035]   
See also in sourсe #XX -- [ Pg.253 , Pg.254 ]



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EcoRl

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