EcoRl sequence

Several unique recognition sequences in pBR322 (Pstl, EcoRl, BamHl, Sail, PvulI) are targets for different restriction endonucleases, providing sites where the plasmid can later be cut to insert foreign DNA. 

Nordland, T. M., Andersson, S., Nilsson, L., Rigler, R., Grasland, A., and McLaughlin, L. W. (1989). Structure and dynamics of a fluorescent DNA oligomer containing the EcoRl recognition sequence Fluorescence, molecular dynamics, and NMR studies. Biochemistry 28, 9095-9103. 

Restriction enzymes are bacterial enzymes that "cut" the sugar-phosphate backbone of DNA molecules at specific nucleotide sequences. The first of these en-z)mes to be purified and studied was called EcoRl. The name is derived from the genus and species name of the bacteria from which it was isolated, in this case E. coli. The following is the specific nucleotide sequence recognized by EcoRl ... 

Figure 5.7-8. Schematic overview of PCR-RFLP. Double-stranded DNA molecules are shown for DNA sample A and B. These two samples are PCR amplicons from genomic DNA. The two sequences differ for the base pair highlighted in red. Sample A contains a restriction enzyme recognition site for EcoRl (underlined), and sample B does not. After restriction enzyme digestion, agarose gel electrophoresis results in different DNA fragments for the two samples. (This figure is available in full color at ftp //ftp.wiley.com/public/ sci tech med/pharmaceutical biotech/.)...

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