Early methodology peptide derivatisation

These procedures were found (Das and Tederer, 1971 Das et al., 1967) to increase the volatility of a peptide by removing some of the intramolecular and inter-molecular polar interactions that are characteristic of amino acids, peptides and proteins. The procedures have continuously been developed during more recent years, since they also form the basis of derivatisation for gas-liquid chromatographic (GTC) analysis of amino acids (Section 4.18.2) and peptides. 

A variety of other derivatisation regimes has been described (Falter, 1971), each with its problems and its advantages. Each has its adherents, but most users of EIMS remain uncommitted to any particular one of them. 

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