Early chick culture

A Method to Culture Early Chick Embryos Andrea Streit... 

Archer C.W., Rooney P., Wolpert L. (1982) Cell shape and cartilage differentiation of early chick limb bud cells in culture. Cell Differentiation, 11 (4), 245-251. 

Most of the early work on the mechanics of gastrulation was performed on the egg of Xenopus laevis, because of its accessibility. New Culture offered a chance to emulate and refine some of those experiments in the chick embryo. 

It was found by Levere et ah [137] that the same 5)6-H steroids that actively stimulated the synthesis of ALA-synthetase in chick embryo liver cells in culture also stimulated hemoglobin synthesis in chick embryo blastoderm cells (Fig. 9). It is inferred that the stimulation by the steroids is brought about by the same mechanism as for the liver cells that is, the steroids induce the synthesis of ALA-synthetase at the transcriptional level. ALA-synthetase is the rate-limiting enzyme in early red cell precursors, and its increase causes an earlier increase in the formation of ALA the ALA, once made, is rapidly converted to heme, and once heme is made globin is synthesized. Thus the controlling step in hemoglobin synthesis appears to be one that is turned on by a 5y -H steroid. Stimulation of hemoglobin synthesis by Sfi-H steroids has recently also been demonstrated in mice and in humans [140,142],... 

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