E2 enzyme

NEDD8 (Rubl in yeast) is a UbL, which is attached to cullins following activation by a specific El and transfer by an E2 enzyme [47]. Cullins are subunits of the SCF... 

Seol, J. H., et al., Cdc53/cullin and the essential Hrtl RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev, 1999, 13(12), 1614-26. 

A protein can be identified as an E2 enzyme according to several different criteria. Functionally, the E2 occupies an intermediate position in the conjugation cascade -... 

Structural biology provides a final way to define an E2 enzyme. As expected from the strong sequence conservation, the E2 core domain adopts a conserved fold. At the time this article was being prepared, twelve different E2 structures had been deposited in the Protein Data Bank. The average root-mean-square deviation of... 

A different kind of relationship between an E2 enzyme and disease is exemplified by the finding that the Alzheimer s amyloid-yS peptide induces the expression of E225K, a mammalian relative of yeast Ubcl [90]. E22sk was found to play a major role in amyloid-j8-dependent neuronal cell killing. This effect may be related... 

E2 Enzymes Dedicated to Ubiquitin-like Proteins (UbLs)... 

Kolman, C. j., Toth, J., and Gonda, D. K. Identification of a portable determinant of cell cycle function within the carboxyl-terminal domain of the yeast CDC34 (UBC3) ubiquitin conjugating (E2) enzyme. EMBO J. 1992, 3 3, 3081-90. 

Fig. 6.2. The structures of SCF, Cul2-Elongin B-C, and Cul3-BTB/POZ ubiquitin E3 ligase complexes with the bound substrates and E2 enzymes.

Elucidation of the structure of the SCF ubiquitin E3 ligase complex and their substrates should help resolve certain issues regarding the mechanism by which SCF E3 ligase promotes ubiquitin transfer from the E2 enzyme to the protein substrate. Recently, the structures of Cull/Rocl in complex with Skpl and Skp2, as well as that of Skpl/jS-Trcp and Cdc4, have been reported [21, 25, 55, 56]. These studies... 

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