Dual pore scaffolds

A simple process for the fabrication of biodegradable dual pore scaffolds for tissue engineering, which have a high surface area and porosity without toxic substance secretion has been presented. Ehral pores may be formed by (47) ... 

A scanning electron microscope (SEM) picture of the external surface of the dual pore scaffolds is shown in Figure 8.6. 

Figure 8.6 External Surface of Dual Pore Scaffolds (47)...

The mixed solution is poured into a frame made of silicone material which is placed in liquid nitrogen. Then, the polymer sample is added to a mixture of water and ethanol and subjected to foaming for 24 h. After foaming, the sample is freeze-dried for 20 h to provide biodegradable dual pore polymer scaffolds. The overall porosity was about 98% which was analyzed by mercury porosimeter. 

D. K. Han, K.-D. Ahn, H.J. Jung, and K. Park, Method of preparing biodegradable dual pore polymer scaffolds for tissue engineering, US Patent 7 842 305, assigned to Korea Institute of Science And Technology (Seoul, KR), November 30, 2010. 

K.M. Brouwer, W.F. Daamen, N. van Lochem, D. Reijnen, R.M.H. Wijnen, T.H. van Kuppevelt, Construction and in vivo evaluation of a dual layered collagenous scaffold with a radial pore structure for repair of the diaphragm, Acta Biomater. 9 (6) (2013) 6844—6851. 

Figure 3. These images represent the types of structural and chemical information that can be obtained using the OCM-CFM dual-mode technique (a,), CARS (b.f and immersive visualization (cf The OCM-CFM images (a) are of fetal chick osteoblasts cultured on porous polycaprolactone scaffolds. Differences in pore size and crystalline regions of the polymer could be determinied at 145 pm from the surface. Figure b is a broadband CARS micrograph of a phase-separated ternary polymer blend. The colors green, blue, and red represent polystyrene, poly(ethylene terphthalate) and poly(methyl methacrylate), respectively. Figure c is a 3D respresentation of cells on polycaprolactone scaffold taken from the immersive visualization laboratory. This visual image provides additional information on cell shape, orientation, and position within the scaffold.

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