Dual-channel imaging technique

Key Words 2D PAGE protein expression profiling DIGE labeling p S]-L-methionine pulse labeling virulence gene expression dual-channel imaging technique. 

Color Plate 3 Dual-channel imaging technique. Bacterial cells were grown in synthetic medium without nitrate to an optical density at 500 nm (OD500) of 0.5. The proteins were labeled with [ S]-L-methionine 20 min after transfer to anaerobic conditions. Protein extracts were separated on 2D gels. The resulting images from the protein synthesis... 

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