Double-sink PAMPA permeability assay

These automated assays can be used for high-throughput ADME screening in early drug discovery. The double-sink PAMPA permeability assay mimics in vivo conditions by the use of a chemical sink in the acceptor wells and pH gradient in the donor wells. The use of the pION gut-box integrated on the deck has shortened the PAMPA assay incubation time to 30 minutes. The permeability coefficient and rank order correlate well with data obtained using the in vitro Caco-2 assay and in vivo permeability properties measured in rat intestinal perfusions. 

Suen Y, Tsinman K, Zhu Z, Threadgill G. Automation of a double-sink PAMPA permeability assay on the Biomek EX Laboratory Automation Workstation. Pharm Discov 2005a, 1 June xx. 

In conclusion, the double-sink su m-P, PAMPA in vitro GIT assay seems to predict human absorption as well as in vivo human permeability measurements (see Figs. 7.66a,b) and in vitro Caco-2 permeability measurements (see Figs. 7.60 and 7.63), but at a lower cost and higher speed. 

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