Dose-response relationship, amino acid

The first step in these studies has been the search for the shortest fragment of the ACTH chain that is essential for (maintenance of) activity. Next, changes in the peptide backbone and modification of the side-chains of the amino acid residues have been studied. As a test system the delay of extinction of an active avoidance response in rats as measured in a pole-jumping test after subcutaneous administration has been used (7 ) this assay method gives a graded dose-response relationship which allows the estimation of an ED50 and thus potency ratio s. The heptapeptide ACTH--(4-10) has been used as the reference peptide (8 ). For a more extensive review see ref. 9. 

Anderson, G. H., Bryan, M. K., Jeejeebhoy, K. N., and Corey, P., 1977, Dose-response relationships between amino acid intake and blood levels in newborn infants. Am. J. Clin. Nutr. 30 1110. 

Rennie, M.J., Bohe, J., and Wolfe, R.R. (2002). Latency, duration and dose response relationships of amino acid effects on human muscle protein synthesis. J Nutr 132 3225S-3227S. 

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