Dojindo Laboratories

Dojindo Laboratories (Japan) Sigma-Aldrich Fine Chemicals... 

Cell inhibition rate analysis Cells were seeded in 96-well plates at a density of 1.5/ 104 cells/well in quadruplicate and incubated at 37°C in a 5% C02 atmosphere. After 24 hr, cells were treated with 0.1% DMSO (control) or, compound at various concentrations (0.01 - 0.33 mM) for 48 hr. Cell inhibition rate was analyzed after 48 hr using the Cell Counting Kit CCK-8 (Dojindo Laboratories, Japan), according to the manufacturer s instructions. OD values were measured at a wavelength of 450 nm using a Multi-Detection Microplate-Reader (Bio-TEK, Winooski, VT). 

N-ethyl-M-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Dojindo laboratories, Kumamoto, Japan). 

The MgO, 99.98% pure, was ultrafine single crystal MgO, 100, furnished by Ube Industries Ltd. The Si02 was prepared by calcination at 800° C of the colloidal silica, Snowtex-N, provided by Nissan Chemical Industries, Ltd. The a-Al2p3 was prepared from Y-AI2Q3 (Showadenko K.K.) by calcination in air at 1200°C for 3 h. M-AA was furnished by Dojindo Laboratories or by Aldrich Chem. Co., Inc. 

Spc troscopic-gtade QHe purchased from Dojindo Laboratories was dried with Molecular Sieves 4A and distilled under a reduced pressure. Dichloromethane-d2 (99.95% up D) obtained from Aldrich Chemical Co., Inc., and benzene-d (99.6% up D) from Wako Pure Chemical Industries, Ltd., were used as received. The molalities of the samples are listed in Table 1. The amount concentrations of the complex solutions were calculated at each temperature from the molalities and the partial molar volumes of the components at infinite dilution. For the CH2Q2 and C6H6 solutions, all the relevant partial molar volumes are available. For the CeD solutions, the partial molar volume of the sdute was assum to be the same as that in CeHe. For the CD2CI2 soluticms, the partial molar volume of the solvent was replaced by that of CH2CI2 and the partial molar volume of the scdute by that in CH2O2. 

Biotinylated luciferase was donated from Kikkoman Co. (Chiba, Japan). D-Luciferin was purchased from Sigma Chemical Co. (St. Louis, MO). Cys-type Aequorin (Cys4-AQ) was donated from Chisso Co. Yokohama Research Center (Kanagawa Japan). N-2-hydroxyethylpiperazine-N -2-ethanesulfonic acid (HEPES) was from Dojindo Laboratories (Kumamoto, Japan). 

N-morpholino) ethanesulfonic acid (MES) (Dojindo Laboratories.) and NaOH were used as buffer agents. Three types of solutions were prepared with specific pH s and ionic strengths. These solutions were of pH 5.6 and ionic strength... 

The binding of antibody to latex particles was performed using the water-soluble carbodiimide (WSC) methodic) with l-ethyl-3-(3-dimethyl-aminopropyl) (EDAC Dojindo Laboratories, Kumamoto, Japan). In this method, carbodiimide activation of carboxylate groups on the surface of latex particles (under acidic conditions) produces an imstable reaction intermediate, O-acylisourea. O-acylisourea subsequently reacts with N-hydroxysuccinimide (NHS Acros Organics, Antwerp, Belgium) to produce a stable NHS ester (Fig. 1). 

Poly(vinyl chloride) (PVC) with an average polymerization degree of 1100 was purified by reprecipitation from THF in methanol. The plasticizers or membrane solvents, di-n-octylphthalate (DOP), o-nitrophenyl octyl ether (NPOE), and 2-fluorophenyl-2-nitrophenyl ether (FPNPE) were obtained from Dojindo Laboratories. The lipophilic salt, sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (NaTFPB), was obtained from Tokyo Chemical Industry Co., Ltd. and Dojindo Laboratories. 

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