Docking motif

No similar tryptophan docking motifs are present in the 7-bladed superbarrel proteins galactose oxidase and methylamine dehydrogenase, or in the 8-bladed nitrite reductase (cytochrome cJi) (Fulop et a/., 1995 Baker et al., 1997). 

Inhibitor-2 has three major PPl-docking motifs. Two of these, the RVxF and SILK-motifs, only serve to anchor PPl, while the docking of the IDoHA a-helix motif to the acidic and hydrophobic grooves blocks access to the active site [25, 34]. Some structural and modeling data suggest that Inhibitor-2 also induces the release of a metal ion from the catalytic site [6, 24, 34]. The Inhibitor-2 induced loss of a metal ion is consistent with a large... 

Hendrickx A, Beullens M, Ceulemans H et al (2009) Docking motif-guided mapping of the interactome of protein phosphatase-1. Chem Biol 16 365-371... 

Meiselbach H, Sticht H, Enz R (2006) Structural analysis of the protein phosphatase 1 docking motif molecular description of binding specificities identifies interacting proteins. Chem Biol 13 49-59... 

Mouse antiphospho PDK docking motif monoclonal antibody (18A2). Use 1 2,000 dilution in TBST containing 5% (w/v) BSA detect with antimouse IgG-HRP. 

Load wells of precast gels with 2.5 pi of sample (4 x 10 cells) for antiphospho PKB substrate antibody and 5 pi (8 x lO cells) for antiphospho PDK docking motif antibody and antiphospho PKC (pan) antibody. 

As shown in Fig. 3b, c, the phospho PDK docking motif antibody, very specifically detects the phosphorylation state of the hydrophobic motif of the PKBRl. This phosphorylation is completely absent in competent cells prior to stimulation. Following addition of cAMP, it increases rapidly, peaks at 30 s, and disappears within 2 min. This phosphorylation is completely absent in cells lacking PiaA and substantially decreased in cells lacking Rip3 (data not shown), indicating that it is a TorC2 substrate. 

---