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DNA synthesiser

The randomised nucleotide library that is used as the input in the initial selection procedure is conveniently generated on a DNA synthesiser and transcribed into an RNA pool by in vitro transcription. Designing the structure of the library is a crucial step which should be planned carefully. Three main types of libraries are in general use A fully randomised library a randomised region which is embedded in a natural RNA structure and a doped library where each position of a specific RNA sequence contains a... [Pg.169]

The enzymatic synthesis of DNA has been described using a circular DNA template. Multiple copies of the template are produced without the requirement for the heating and cooling cycles associated with PCR. The concatermeric products can be cleaved with enzymes to yield shorter multiple copies of oligonucleotides. An automated multiplex DNA synthesiser has been developed which can simultaneously and rapidly synthesise up to 96 different oligonucleotides. ... [Pg.194]

See other pages where DNA synthesiser is mentioned: [Pg.569]    [Pg.532]    [Pg.408]    [Pg.1179]    [Pg.1179]    [Pg.206]    [Pg.206]    [Pg.207]    [Pg.404]    [Pg.532]    [Pg.46]    [Pg.48]    [Pg.613]    [Pg.661]    [Pg.18]    [Pg.553]    [Pg.221]    [Pg.223]    [Pg.627]    [Pg.804]    [Pg.804]    [Pg.1002]    [Pg.148]   
See also in sourсe #XX -- [ Pg.19 , Pg.539 ]



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Synthesised

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