DNA sequencing machines

In recent years, automated DNA sequencing machines capable of identifying about 10 bases per day have become commercially available. One clever innovation has been the use of fluorescent dyes of different colors to uniquely label the primer DNA introduced into the four sequencing reactions for example, red for the A reaction, blue for T, green for G, and yellow for C. Then, all four reaction mixtures can be combined and run together on one electrophoretic... 

Until the development of automated DNA sequencing machines in the 1990s, two techniques were used to sequence the bases in a segment of DNA. Each of the techniques involves the isolation of a restriction fragment containing a few hundred or a few thousand base pairs. The DNA is denatured and each strand is sequenced separately so... 

A variation on this method is to use a single reaction mixture with each of the four ddNTPs labeled with a different fluorescent indicator. Each label is then detected by its characteristic spectrum following separation of the fragments by polyacrylamide gel electrophoresis or some chromatography method. First-generation automated DNA sequencing machines use this fluorescent variation. 

DNA sequencing machines. In addition, compared to HTS, DEL demonstrates significant advantages in compound storage and cost-effectiveness. 

Equipment PCR machine, scintillation counter, tabletop centrifuge, temperature-controlled water baths, equipment for horizontal and vertical electrophoresis, UV-illuminator, phosphor imager, automatic DNA sequencer, vacuum dot-blot manifold (Schleicher and Schuell). PCR 0.5 ml hot-start mbes, aerosol resistant pipette rips, autoclaved Eppendorf tubes (all from Fischer Scientific, Brightwaters, NY) and glassware, diethyl pyrocarbonate (DEPC, Sigma)-treated solutions. 

Lapedes, A., Barnes, C Burks, C., Farber, R. Sirotkin, K. (1989). Application of neural networks and other machine learning algorithms to DNA sequence analysis. In Computers and DNA, SFI Studies in the Sciences of Complexity, vol. 7 (ed. Bell, G. I. Marr, T. G.), pp. 157-82. Addison-Wesley, Rosewood City, CA. 

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