DNA sequenator

Soper, S.A., Williams, D.C., Xu, Y., Lassiter, S.J., Zhang, Y., Ford, S.M., Sanger DNA-sequeneing reactions performed in a solid-phase nanoreactor directly coupled to capillary gel electrophoresis. Anal. Chem., 70,4036, 1998. 

He, Y, Pang, H.-M., and Yeung, E.S., Integrated electroosmotically-driven on-line sample purification system for nanofiter DNA sequeneing by capillary electrophoresis, J. Chromatogr. A, 894,179, 2000. 

A modification of Sanger s method has resulted in the commercial availability of automated DNA sequenators based on Sanger s use of dideoxy analogs of nucleotides. Instead, however, of tagging a primer with P, the purine and pyrimidine base portions of the dideoxynucleotides are each modified to contain a side chain that bears a different fluorescent dye, and all the dideoxy analogs are present in the same reaction. After electrophoretic separation of the products in a single lane, the gel is... 

Figure 2. A) Simplified block diagram of automated DNA seque-nator. B) Idealized output from automated DNA sequenator.

Most DNA, natural and synthetic, can adopt the B-form as defined by its characteristic X-ray pattern [3]. There are few a species of synthetic DNA, namely poly(dA) poly(dT), poly(dI) poly(dC), poly(dA-dI) poly(dC-dT) and poly(dI-dC)-poly(dI-dC), which have a B-form that significantly differs from that of the other complementary deoxy polymers due to their different intramolecular packing arrangements and slightly changed value for the rise per residue. The helix parameters are presented in Table 1. The linear sequen-... 

Protein phosphatases 544, 646 Protein S 634 Protein sequenators 118 Protein sequences from genes 119 Protein synthesis 3, 538, 539 Protein tyrosine kinases 544 Protein-disulfide isomerase 83 Protein-DNA interactions 266 Proteinase. See Protease Proteoglycan(s) 181,182. See also Glycosami-noglycans... 

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