Proteins sequenator

The two membranes most used for protein work are nitrocellulose and polyvi-nylidene fluoride (PVDF). Both bind proteins at about 100 pg/cm2. Nitrocellulose is the best membrane to use in the initial stages of an experiment. PVDF is used when proteins are to be sequenced or placed into a (matrix-assisted laser desorption ionization) mass spectrometer. PVDF can withstand the harsh chemicals of protein sequenators and the heat generated by mass spectrometer lasers, whereas nitrocellulose cannot. 

Ingenious protein sequenators have been devised to carry out the Edman degradation automatically.242 244-246 Each released phenylthiohydantoin is then identified by HPLC or other techniques. Commercial sequenators have often required 5-20 nmol of peptide but new microsequenators can be used with amounts as low as 5-10 picomoles or less.247 248... 

Protein phosphatases 544, 646 Protein S 634 Protein sequenators 118 Protein sequences from genes 119 Protein synthesis 3, 538, 539 Protein tyrosine kinases 544 Protein-disulfide isomerase 83 Protein-DNA interactions 266 Proteinase. See Protease Proteoglycan(s) 181,182. See also Glycosami-noglycans... 

R.E. Lovins, J. Craig, F. Thomas, C.R. McKinney, Quantitative protein sequencing using MS A protein-sequenator-MS interface employing flash evaporative techniques. Anal. Biochem., 47 (1972) 539. 

Edman P and Begg G (1967) A protein sequenator. European Journal of Biochemistry 1 80-90. 

Hunkapiller MW, Hood LE, and Dreyer WJ (1981) A gas liquid solid phase peptide and protein sequenator. Journal of Biological Chemistry 256 7990-7997. 

Niall HD (1973) Automated Edman degradation. The protein sequenator. In Hirs GHW and Timashoff SN (eds.) Methods in Enzymology, pp. 179-238. New York Academic Press. 

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