DNA oligonucleotides

Sauer M, Drexhage K H, Lieberwirth U, Muller R, Nerd S and Zander C 1998 Dynamics of the electron transfer reaction between an oxazine dye and DNA oligonucleotides motored on the single-molecule level Chem. Phys. Lett. 284 153-63... 

Antisense therapy means the selective, sequence-specific inhibition of gene expression by single-stranded DNA oligonucleotides. By hybridizing to the target mRNA, which results in a subsequent double-helix formation, gene expression is blocked. This process can occur at any point between the conclusion of transcription and initiation of translation or even possibly during translation. 

The electrophoretic mobilities of flexible macromolecnles (e.g., DNA, oligonucleotides, and other polymers) in gel media have also been extensively stndied by a nnm-ber of methods, including Monte Carlo simnlations [159,165,208,357,358,361,362,447]. In general, the mobility is expected to vary with the length of the polymer to the -1 power (p N y, however, there are complicating effects of the applied electric field as well as the... 

Nicolau DV, Sawant PD (2005) Scanning Probe Microscopy Studies of Surface-Immobilised DNA/Oligonucleotide Molecules. 260 113-160 Niessen HG, Woelk K (2007) Investigations in Supercritical Fluids. 276 69-110 Nilsson P, Olofsson K, Larhed M (2006) Microwave-Assisted and Metal-Catalyzed Coupling Reactions. 266 103-144... 

Therefore, the sequence of reactions illustrated in Fig. 1 catalytically (the anthraquinone is regenerated) injects a radical cation into a DNA oligonucleotide that does not simultaneously contain a radical anion. As a result, the lifetime of this radical cation is determined by its relatively slow bimolecular reaction with H20 (or some other diffusible reagent such as 02- ) and not by a rapid intramolecular charge annihilation reaction. This provides sufficient time for the long distance migration of the radical cation in DNA to occur. 

V. Chan, S.E. McKenzie, S. Surrey, P. Fortina, and DJ. Graves, Effect of hydrophobidty and electrostatics on adsorption of DNA oligonucleotides at liquid/solid interfaces. J. Coll. Interf. Sci. 203, 197-207 (1998). 

Determining the thermodynamics and kinetics of recognition between biomolecules, particularly when one of the two is immobilized on a substrate, is of considerable current interest, as for example, antibody-antigen recognition, recognition of single-stranded DNA oligonucleotides by partially or totally complementary DNA strands, and many other possible analytical applications. 

Fig. 2 (a) Schematic of the formation of silver clusters using DNA oligonucleotide as scaffold. After complexation of DNA with silver cations, the mixture is reduced with NaBtL, and the fluorescent cluster is formed, (b) Absorption spectra of silver clusters acquired every 30 min using [5 -AGGTCGCCGCCC-3 ] = 10 uM. [Ag+] = 60 uM. and [BfLj-] = 60 pM. The foremost spectrum was acquired 9 min after adding the BH4, and it has at 426 nm. The inset spectrum shows the last spectmm in the series (692 min), with peaks at 424 and 520 nm. (c) Induced circular dichroism spectra. The cell path length was 5 cm. The spectra were collected 2 min (A, dashed-dotted line), 20 min (B, dotted line), 40 min (C,fine dashed line), 60 min (D, coarse dotted line), and 150 min (E, solid line) after adding the BH [32]... 

Baker SE, Cai W, Lasseter TL, Weidkamp KP, Hamers RJ (2002) Covalently bonded adducts of deoxyribonucleic acid (DNA) oligonucleotides with single-wall carbon nanotubes Synthesis and hybridization. Nano Lett. 2 1413-1417. 

Gao HJ, Kong Y, Cui DX, Ozkan CS (2003) Spontaneous insertion of DNA oligonucleotides into carbon nanotubes. Nano Lett. 3 471 173. 

Fig. 9.1 Simulation snapshots of a DNA oligonucleotide (8 adenine bases) interacting with a (10,10) carbon nanotube at 0, 30, 100, and 500 ps. Water molecules are not displayed for clarity (Chaudhary et al., 2006. With permission from American Chemical Society) (See Color Plates)...

Presented here is a concise description of surface immobilization of DNA, oligonucleotides, and DNA derivatives by adsorption onto carbonaceous materials, and the properties of the DNA layer adsorbed on carbonaceous solid phase. 

Scanning Probe Microscopy Studies of Surface-Immobilised DNA/Oligonucleotide Molecules... 

The ability to synthesize chemically short sequences of single-stranded DNA (oligonucleotides) is an essential part of many aspects of genetic engineering. The method most frequently employed is that of solid-phase synthesis, where the basic philosophy is the same as that in solid-phase peptide synthesis (see Section 13.6.3). In other words, the growing nucleic acid is attached to a suitable solid support, protected nucleotides are supplied in the appropriate sequence, and each addition is followed by repeated coupling and deprotection cycles. 

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