DNA nanostructure

Figure 3.24 Dynamic combinatorial selection of DNA nanostructure (Aldaye and Sleiman).

Fig. 23 AFM images of examples of calculations by DNA self-assembly, a Section of patterned DNA lattice containing barcode information of 01101 the 1 and Obit values are clearly visible as lighter and darker stripes, respectively (from [151] reprinted with permission), b Branched tiled DNA nanostructures, mimicking the solution of a Pascal triangle [152]. Reprinted with permission...

ENVIRONMENTAL EFFECTS INFLUENCING THE VIBRATIONAL MODES OF DNA NANOSTRUCTURES COUPLED TO BIOMOLECULES... 

Reif, J.H., Labean, T.H., Seeman, N.C., 2001. Challenges and applications for self-assembled DNA nanostructures DNA Computing. Springer. 

In this chapter, attention is focused on novel developments for the production of pristine nucleic acid architectures and DNA-polymer hybrid structures, employing the methods, techniques, and materials acquired from molecular biology. However, while the details of linear DNA block copolymers (DBCs), DNA networks, and catenated DNA structures will be included, the preparation and modification of regular DNA nanostructures, using molecular biology methods and enzymes, will not be discussed as an excellent summary of these procedures is available elsewhere [15]. 

Chow DC, Lee WK, Zauscher S, Chilkoti A (2005) Enzymatic fabrication of DNA nanostructures extension of a self-assembled oligonucleotide monolayer on gold arrays. J Am Chem Soc 127 14122-14123... 

Fig. 7.6 Molecular orbital energies (eV) for the CNT-DNA nanostructure in a water and b vacuum conditions. Highlighted squares indicate HOMO and LUMO of the complex system. Green ellipses indicate carbon nanotube HOMO and LUMO. Orbitals are localized in the carbon nanombe (CNT), water molecules (water), bases (Base), and Phosphate groups (Phosphate) [53]...

Rate-zonal centrifugation may be used for preparation of larger amounts of DNA nanostructures. 

Click chemistry has proven to be a valuable tool to stabilize self-assembled nucleic acid nanostructures by covalent cross-links that are more stable to denaturing agents, or can, for example, be freeze-dried. Discussing various aspects of nanostructure stabilization will go beyond the scope of this chapter. Thus, only selected examples involving click chemistry for DNA nanostructure assembly and stabilization are presented. 

Hexagonal DNA modules have been self-assembled and stabiUzed via CuAAC click chemistry by six simultaneous reactions, a method which is applicable for stabilization of various DNA nanostructures [176]. 

DNA nanopatterns on surfaces have been immobilized using click chemistry [177], and branched, Y-shaped DNA molecules can be prepared from tripropar-gylated oligonucleotides [178] by CuAAC click reactions, which are useful building blocks for higher DNA nanostructures. Moreover, SPAAC click chemistry in combination with orthogonal photochemical fixation has been used to synthesize oligomeric DNA scaffolds from cyclic DNA nanostructures which are stable towards denaturation and allow facile purification [179]. 

Cassinelli V, Oberleitner B, Sobotta J, Nickels P, Grossi G, Kempter S, Frischmuth T, Liedl T, Manetto A (2015) One-step formation of chain-armor -stabilized DNA nanostructures. Angew Chem 54(27) 7795-7798. doi 10.1002/anie.201500561... 

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