DNA-encoded antibody libraries

Bailey RC, et al. DNA-encoded antibody libraries a unified platform for multiplexed cell sorting and detection of genes and proteins. J Am. Chem. Soc. 2007 129 1959-1967. 

In a model enrichment, the DNA encoding a peptide with the myc-epitope was diluted in different ratios in a DNA pool encoding random peptide sequences. The myc-epitope peptide was enriched by immuno-precipitation with an anti-c-myc antibody by a factor of twenty to forty per cycle of mRNA-peptide fusion (Roberts and Szostak, 1997). More recently, the mRNA-peptide fusion approach has been used to select peptides for binding to this anti-c-myc antibody from a library of 2 X 1013 molecules (Roberts andja, 1999). In these experiments, the enrichment factor was reported to be 200 per round and is therefore similar to the enrichment factors observed with ribosome display. 

To obtain the sequence for CBP, a cDNA library from the silk gland was prepared and screened using a anti-CBP antibody. One positive clone was identified from 200,000 plaques, and rapid amplification of 5 complementary DNA ends (5 -RACE) was performed to obtain the full length of the CBP cDNA (Figure 24.2a). The predicted sequence encodes a 297-residue polypeptide of... 

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