DNA cationic lipids

Golubovic, L. and Golubovic, M. (1998) Fluctuations of quasi-two-dimensional smectic intercalated between membrane in multilamellar phases of DNA-cationic lipid complexes. Phys. Rev. Lett., 80,4341—4344. 

Bminsma, R. (1998) Electrostatics of DNA cationic lipid complexes isoelectric instability. Eur. Phys. J. B, 4, 75-88. Bminsma, R. and Mashl, J. (1998) Long-range electrostatic interaction in DNA cationic lipid complexes. Europhys. Lett., 41(2), 165-170. 

Salditt, T., Koltover, I., Raedler, J.O. and Safinya, C.R. (1998) Self-assembled DNA-cationic lipid complexes two-dimensional smectic ordering, correlations, and interactions. Phys. Rev. E, 58, 889-904. 

Boukhnikachvili, T., Aguerre-Chariol, O., Airiau, M., Lesieur, S., Ollivon, M. and Vacus, J. (1997) Structure of inserum transfecting DNA-cationic lipid complexes. FEES Lett., 409, 188-194. 

Boukhnikachvili T, AguerreChariol O, Airiau M et al (1997) Structure of in-serum transfecting DNA-cationic lipid complexes. FEBS Lett 409 188-194... 

Bruinsma R (1998) Electrostatics of DNA cationic lipid complexes isoelectric instability. Eur Phys J B 4 75-88... 

Fig. 6 Schematic representation of a complex of DNA-cationic lipid, and b DNA and a mixture of cationic lipid and neutral lipid, the lipid-DNA complexes intercalated in multilamellar membranes of cationic lipid and neutral lipid. Part b was reproduced with permission from [39]...

Both protamine sulfate and PLL were compared for their transfection efificiencies. With the same amount of DNA and cationic lipid, PLL reached its efficiency plateau at an amount one-half the amount of protamine sulfate, but the overall efficiency of protamine sulfate was two- to sevenfold higher than PLL in different cell lines. The extent of transfection efficiency for LPD-I was 7- to 45-fold higher than the levels shown by DNA/cationic lipid complex. Phosphate and free base form of protamine showed lower levels of transfection and the activity was comparable with lipoplex. It is noteworthy that arginine, which exists as a salt of sulfate, and the lysine as free base or as a phosphated salt, showed a variance in the relative transfection efificiencies even though the percentage of basic (arginine + lysine) residues remained relatively constant. 

Osaka, G., Carey, K., Cuthbertson, A., Godowski, P Patapoff, T., Ryan, A., Gadek, T., and Mordenti, J. (1996) Pharmacokinetics, tissue distribution, and expression efficiency of plasmid [33P]DNA following intravenous administration of DNA/cationic lipid complexes in mice—use of a novel radionuclide approach. J. Pharm. Sci. 85(6), 612-618. 

Parker, S. E., Ducharme, D., Norman, J., and Wheeler, C. J. (1997) Tissue distribution of the cytofectin component of a plasmid DNA/cationic lipid complex following intravenous administration in mice. Hum. Gene Ther. 8(4), 393 401. 

Although not commonly discussed, the dependence of the sample preparation protocols on the results has been known for a long time. As an example, protocols for lipoplex (DNA—cationic lipid complexes) preparation describe the order of addition of the different components according to their concentration so that the neutralization (and consequence precipitation) line in the phase diagram is not crossed [46]. 

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