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Disulfides with 2-mercaptoethylamine

Reduction of the cystamine-labeled oligo using a disulfide reducing agent releases 2-mercaptoethylamine and creates a thiol group (Fig. 399). DNA probes labeled in this manner have been successfully conjugated with SPDP-activated alkaline phosphatase (Chapter 16, Section 1.5, and Section 2.4), maleimide-activated horseradish peroxidase (section 3.8.1), NHS-LC-biotin (Chapter 8, Section 3.1, and Section 2.3), and the fluorescent tag AMCA-HPDP (Chapter 8, Section 1.3, and Section 2.5). [Pg.671]

The reaction of cyclohexanone with bis(2-aminoethyl)disulfide, under nitrogen and in the presence of toluene-p-sulfonic acid, afforded the dihy-drothiazine 117 and 2-mercaptoethylamine, probably by way of the intermediate 116 cyclooctanone, 2,6-dimethylheptan-4-one, and isopropyl phenyl ketone behaved similarly. ... [Pg.321]

Numerous reducing agents have been applied for the cleavage of disulfides in peptides. Reduction with sodium in liquid ammonia, with cysteine, 2-mer-captoethanol, 2-mercaptoacetic acid, 2-mercaptoethylamine (cysteamine), borohydrides and trialkylphosphines were used both for preparative and for... [Pg.36]

See other pages where Disulfides with 2-mercaptoethylamine is mentioned: [Pg.342]    [Pg.84]    [Pg.88]    [Pg.786]    [Pg.93]    [Pg.96]    [Pg.479]    [Pg.481]    [Pg.483]    [Pg.501]    [Pg.502]    [Pg.109]    [Pg.228]    [Pg.59]    [Pg.263]    [Pg.73]    [Pg.76]    [Pg.459]    [Pg.461]    [Pg.463]    [Pg.481]    [Pg.482]    [Pg.783]    [Pg.452]    [Pg.783]    [Pg.1174]    [Pg.325]    [Pg.37]    [Pg.102]    [Pg.239]    [Pg.325]   
See also in sourсe #XX -- [ Pg.94 ]



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Disulfide 2-mercaptoethylamine

Mercaptoethylamines

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