Dissociation separation

Base A substance which dissociates (separates) in aqueous solution to yield hydroxyl ions, or one containing hydroxyl ions (OH-) which reacts with an acid to form a salt or which may react with metal to form a precipitate. 

Separation (dissociation) separation of the copy from the matrix (this is carried out by washing with dilute sodium hydroxide). 

Rinsing the sensor surface with buffer results in an irreversible dissociation, because all molecules which dissociate from immobilized protein are removed from the system by the buffer stream, allowing one to determine the rate constant of dissociation separately. 

MX. In Process I, M+ and XT ions, which are strongly bound electrostatically in the crystal, are dissociated (separated from one another) and are brought into a gaseous state. In Process II, the M+ and XT ions in the gas phase dissolve into the solvent by being solvated. In Process III, the crystal of MX directly dissolves into the solvent, forming the solvated M+ and XT ions. The Gibbs energies for the three processes are related as follows ... 

Self-Test 8.12A How many moles of ions are present in a solution containing 0.10 mol Na2S04, assuming complete dissociation (separation of ions) Estimate the i factor. 

In our laboratory, we have developed a novel MALDI-MS based assay in combination with separation techniques to rapidly identify and confirm the presence of viral proteins (immature or mature) and impurities in the rAd vector [138], The approach combines powerful multidimensional analytical techniques to fully characterize viral proteins, including RP-HPLC, SDS-PAGE, MALDI-MS, MS/MS, and database searching methods (Figure 19-24). This assay involves dissociation/separation of intact viruses by RP-HPLC, separation of the SDS-dissociated viruses by SDS-PAGE, and enzymatic digestion of the dissociated viral proteins from the RP-HPLC fractions and the gel bands, followed by MALDI-MS, MALDLpost source decay (PSD) studies, and database search. 

The subunit domain a b2 Ci2 in Fq is considered to be the site for proton translocation, but the details of the mechanism remain to be explored. It is generally agreed, however, that the a- and c-subunits mediate proton translocation, while the b-subunit only acts as a structural element to link F, (a3 p3) with Fq (the a-subunit). It is also known from the study of Schneider and Altendorf that all three subunits, a, b and c, are required for an active proton channel reconstituted in E. coli ATP synthase. In their experiment, these authors first dissociated, separated and purified the individual subunits and then integrated the subunits in all possible combinations into phospholipid vesicles. Each assembly was then tested for proton-translocation activity as well as its ability to bind to Fi, and it was found that functional activity could only be achieved by the combination a b2 Cio, the same combination that exists in native Fq. 

Dissociation Separating self from one s own experience Fugue, depersonalization, amnesia, multiple personality... 

The operating pressure in this MP recirculation stage is about 20 bars. After the urea solution leaves the MP dissociation separator, it flows into an adiabatic CO stripper, which uses CO to strip the solution. 

At high temperatures, the halogen FRs (H-FRs) first must dissociate separating the halogen radicals from the molecule s organic component. 

Holstein, S. E. H., Ungewickell, H., and Ungewickell, E. (1996). Mechanism of clathrin basket dissociation Separate functions of protein domains of the DnaJ-homologue auxilin. J. Cell Biol. 135, 925-937. 

Solutes can be classified by their ability to conduct an electrical current. When electrolytes dissolve in water, the process of dissociation separates them into ions forming solutions that conduct electricity. When nonelectrolytes dissolve in water, they do not separate into ions and their solutions do not conduct electricity. 

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