Displacement binding

Displacement binding can be done with any molecule and measures the interference of the molecule with a bound tracer. 

Analogous to competitive displacement binding, agonist [A] and antagonist [B] compete for receptor (R) occupancy ... 

Cherny D.Y., Belotserkovskii B.P., Frank-Kamenetskii M.D., Egholm M., Buchardt O., Berg R. H., Nielsen, P.E. DNA unwinding upon strand-displacement binding of a thymine-substituted polyamide to double-stranded DNA. Proc. Natl Acad. Sci. USA 1993 90 1667-1670... 

Displacement binding experiments with 3H-PCP were conducted with minor modifications of the filtration method described in the literature (Zukin and Zukin 1979 Vincent et al. 1979 Hampton et al. 1982) in crude tissue preparations of rat whole brain homogenates in 5 mM Tris-HCl (pH 7.4). 

Table Z3 Thermodynamic parameters for [3H]-MRE 3008F20 displacement binding experiments to hA/lHO cells...

Rodgers, G. et al. 2003. Development of displacement binding and GTPy scintillation proximity assays for the identification of antagonists of the micro-opioid receptor. Assay Drug Dev. Technol. 1, 627-636. 

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