1,2-dioleoyl-3-trimethylammonium propane DOTAP

Egg phosphatidylcholine (EPC), cholesterol (Ch), mPEG ggg-DSPE, l,2-dioleoyl-3-trimethylammonium-propane (DOTAP), Rhodamine-PE (Rh-PE), and phosphatidylthio-ethanolamine (DPPE-SH) (all from Avanti Polar Lipids). 

Dioleoyl-l,2-diacyl-3-trimethylammonium propane (DOTAP) can be obtained from Avanti Polar Lipids (Alabaster, AL) or as methyl sulfate salt from Sigma (St. Louis, MO). 

Liposomes. Liposomes are vesicles that consist of an aqueous compartment enclosed in a phospholipids bilayer. As they lack proteinaceous components, liposomes are not immunogenic. A broad variety of cationic liposomes containing eventually modified lipids have been used to deliver pDNA and mRNA for therapeutic utilizations. These formulations are usually based on a cationic lipid such as l,2-dioleol-3-trimethylammonium propane (DOTAP) and a fusogenic lipid such as l,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) [22,23]. Despite their ease of production and efficacy in vitro for transfecting cultured cells, cationic liposomes face toxicity issues in vivo [24,25]. Meanwhile, their heterogeneous size... 

Fig. 2 Chemical structures of the zwitterionic neutral lipids DOPC (1,2-dioleoyl-.s -glycero-3-phosphatidylcholine) and DOPE (l,2-dioleoyl-S7J-glycero-3-phosphatidylethanolamine) and the cationic lipids DOTAP (l,2-dioleoyl-3-trimethylammonium-propane, a UVL) and MVL5 (a custom-synthesized MVL)...

---