Dioleoyl-sn-glycero-3-phosphoethanolamine

DOPE l,2-Dioleoyl-sn-glycero-3-phosphoethanolamine DPPC l,2-Dipalmitoyl-SM-glycero-3-phosphocholine DPPS l,2-Dipalmitoyl-s -glycero-3-phosphoserine LB Langmuir-Blodgett MC Monte Carlo... 

Liposomes. Liposomes are vesicles that consist of an aqueous compartment enclosed in a phospholipids bilayer. As they lack proteinaceous components, liposomes are not immunogenic. A broad variety of cationic liposomes containing eventually modified lipids have been used to deliver pDNA and mRNA for therapeutic utilizations. These formulations are usually based on a cationic lipid such as l,2-dioleol-3-trimethylammonium propane (DOTAP) and a fusogenic lipid such as l,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) [22,23]. Despite their ease of production and efficacy in vitro for transfecting cultured cells, cationic liposomes face toxicity issues in vivo [24,25]. Meanwhile, their heterogeneous size... 

FIGURE 6.24 Examples of SAXS data collected in two different lipid lamellar phases, (a) The gel phase Lp. on a sample of pure DPPC (l,2-dlpalmltoyl-sn-glycero-3-phosphocholine) and (b) in the liquid crystalline lipid phase on a sample of pure DOPE (l,2-dioleoyl-sn-glycero-3-phosphoethanolamine. Both sets of data were collected using a synchrotron x-ray source at Brookhaven National Laboratory. 

Dissolve in chloroform 196 mg of egg phosphatidylcholine, 45 mg of cholesterol, 5 mg of l,2-distearoyl-sn-glycero-3-phosphoethanolamine-M-[methoxy(polyethylen-eglycol)-2000] and 7. 5 mg of l,2-dioleoyl-3-trimethyl-ammonium-propane. 

Vesicles were swollen from 1,2-dioleoyl-s/i-glycero-3-phosphocholine (Avanti Polar Lipids, USA) and V- (6-(biotinoyl)amino)hexanoyl)-l,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE, Molecular Probes, The Netherlands) at a ratio of 99 1. Tubes were grown out of lipid globules in shear flow and cut to the desired length with a pipette. Streptavidin-coated beads (Dynal, Germany) were used... 

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