1, 1 -dioctadecyl-3, 3, 3 , 3 tetramethylindocarbocyanine

Stock solutions containing fluorescent labels DiD (l,l -dioctadecyl-3,3,3, 3 -tetramethylindocarbocyanine 4-chlorobezenesulfonate salt) (Molecular Probes, Eugene, OR, USA) or Lissamine rhodamine B labelled glycerophos-phoethanolamine (Avanti Polar Lipids, Alabaster, USA) are made in ethanol and kept at -20°C until use. These lipids are used to label the bilayer of the liposomes. 

The technique was demonstrated for the dyes rhodamine 6G (R6G), sulforho-damine B (SRB), dibenzanthranthene (DBATT), and l,l -dioctadecyl-3,3,3, 3, -tetramethylindocarbocyanine perchlorate (Dil). Figure 5.128 shows the probability of identifying any one of the dyes versus the number of recorded photons for pure lifetime classification (left) and combined lifetime and wavelength elassifieation (right). 

Other Names 3//-Indolium, 2-[3-(l,3-dihydro-3,3-dimethyl- 1 -octadecyl-2//-indol-2-ylidene)-1 -propenyl]-3,3-dimethyl-l-octadecyl, perchlorate l,l -Dioctadecyl-3,3,3, 3 -tetramethylindocarbocyanine perchlorate D 282 D 282 (dye) Dil Dil (dye) DilC18(3)... 

Abbreviations Choi, cholesterol Dil, l,l -dioctadecyl-3,3,3, 3 -tetramethylindocarbocyanine perchlorate DPPC, L-a- dipalnritoylphosphiatirtylcholine MPEG-CHO, mono-methoxypoly (ethylene glycol)5000-aldehyde MPEG-DSPE, monomethoxypoly (ethylene glycol)2(XX)-succinyl phosphatidyl-ethanolanune PBS, phosphate bulferedsahne PMN, polymorphonuclear leukocyte SA, stearylamine SHz, stearic hydrazide. 

Aberrations can be introduced by passing the light through a water layer before it reaches the sample. With this technique, molecules of which the transition dipole moment is oriented along the optical axis appear to be out of focus, while molecules oriented in the sample plane are observed to be in focus. The full 3D orientation can be determined by periodically rotating the laser polarization. The rotation of DilCig (l,T-dioctadecyl-3,3,3, 3 -tetramethylindocarbocyanine perchlorate) in PMMA has been observed using this technique. The timescale of the rotational diffusion was found to be several hundreds of milliseconds. 

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