4,4’ -Diisothiocyanatostilbene-2,2’ disulfonic acid

Several compounds that inhibit vesicular glutamate transport have been identified These include the dyes Evans Blue and Rose Bengal. In addition, the stilbene derivative 4,4 -diisothiocyanatostilbene-2,2 -disulfonic acid (DEDS), a compound commonly used as a specific inhibitor of anion channels, inhibits vesicular glutamate transport. Most known inhibitors have limited use as they are membrane impermeant, with the exception of Rose Bengal. 

Picher etal, 1994). This ATPase could be substantially ( 70% in rabbit portal vein) reduced by pretreatment of the preparation by the ectonucleotidase inhibitor 4,4 -diisothiocyanatostilbene-2,2 -disulfonic acid (DIDS), followed by inclusion of sodium azide in solutions used for permeabilized preparations. This treatment dramatically reduced the ADP/ATP ratio measured in permeabilized muscle and increased contractile force. This method may greatly improve the usefulness of a-toxin-permeabilized preparations for energetics studies. 

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