Diisopropyl fluorophosphonate

Little is yet known in regard to factors that might cause individual variation in acetylcholinesterase activity. The activity is greatly reduced in erythrocytes of persons with paroxysmal nocturnal hemoglobinuria, but this condition is rare (42). It has been reported that extended inhibition by organophosphates does not appear to affect adversely the survival of erythrocytes in the circulation as evidenced by observations (28,60,92) with DFP (diisopropyl fluorophosphonate), OMPA (octa-methyl pyrophosphoramide), and malathion (O,O-dimethyl dithiophos-phate of diethyl mercaptosuccinate). 

SYNS DFP DIFLUPYL DIFLUROPHATE DIISOPROPOXYPHOSPHORYL FLUORIDE DIISOPROPYL FLUOROPHOSPHATE 0,0-DIISOPROPYL FLUOROPHOSPHATE DIISOPROPYL FLUOROPHOSPHONATE DIISOPROP i LFLUORO-PHOSPHORIC ACID ESTER DIISOPROPYLFLUOR-PHOSPHORSAEUREESTER (GER.VIAN) DIISOPROPYL PHOSPHOFLUORIDATE DIISOPROPYL PHOSPHOROFLUORIDATE 0,0 -DIIS0PR0PYL PHOSP-HORYL FLUORIDE DYFLOS FLOROPRYL FLUOPHOSPHORIC ACID, DIISOPROPYL ESTER FLUORODIISOPROPYL PHOSPHATE FLUOROPRYL... 

O, 0-DIIS0PR0PYL FLUOROPHOSPHATE see IRFOOO DIISOPROPYL FLUOROPHOSPHONATE see IRFOOO DIISOPROPYLFLUOROPHOSPHORIC ACID ESTER see IRFOOO... 

Hawkins and Reifenrath made in vitro percutaneous absorption measurements using neat compoimds applied to skin and allowed to evaporate to air circulated over the apphed dose. For studies on benzo(a)pyrene absorption without sodium azide (NaNj), they foimd that mouse skin (6% of dose absorbed) was more permeable than human skin (1% of dose absorbed), which was more permeable than pig skin (0.6% of dose absorbed). In a similar study using 11 compounds [DDT, benzo(a)pyrene, fluocinonide acetonide, progesterone, hndane, testosterone, par-athion, diisopropyl fluorophosphonate, malathion, benzoic acid, and caffeine], they found that there was no statistically significant difference between human and pig skin. 

Adrian et al. 1946 also E.D. Adrian, W Feldberg, B.A. Kilby, and M. KUby, Report XZ71 on Dimethyl Fluorophosphonates to the MoS , 8 October 1941 A.A. Barrett, W Feldberg, B.A. Kilby, and M. Kilby, Report XZIIi on Physiological Examination of Diisopropyl Fluorophosphonates to the MoS , 10 November 1942 both reports in Adrian et al. 1946. 

Myers, D.K., 1952. Competition of the aliesterase in rat serum with the pseudo cholinesterase for diisopropyl fluorophosphonate. Science 115,568-570. 

The power of the pooled GST fusion protein approach will increase as new biochemical reagents and assays become available. The development of chemical probes for biological processes, termed chemical biology, is a rapidly advancing field. For example, the chemical synthesis of an active site directed probe for identification of members of the serine hydrolase enzyme family has recently been described (Liu et al., 1999). The activity of the probe is based on the potent and irreversible inhibition of serine hydrolases by fluorophosphate (FP) derivatives such as diisopropyl fluorophosphate. The probe consists of a biotinylated long-chain fluorophosphonate, called FP-biotin (Liu et al., 1999). The FP-biotin was tested on crude tissue extracts from various organs of the rat. These experiments showed that the reagent can react with numerous serine hydrolases in crude extracts and can detect enzymes at subnanomolar... 

Chemical name Ethyl dimethylamido cyanophosphate Isopropyl methyl pho.sphonofl uoridate Pinacolyl methyl phosphonoflooridate U-cy clohcx yl methyl fluorophosphonate 5-(2-diisopropyl-aminoeth>i) 0-ethyl methyl phosphonothiolate... 

---