Differential interference contrast spectroscopy

Suci et al. [918] have demonstrated that the possibilities of ATR can be extended by combining the method with reflected differential interference contrast spectroscopy. It was shown that such an approach allows the biofihn structure, distribution, and chemistry at solid-liquid interfaces to be investigated simultaneously, the data being obtained in situ, quasi-simultaneously, nondestructively, and in real time. 

Infrared microscopes are commercially available from a number of companies worldwide, and operate much like conventional visible light microscopes. The IR radiation follows the same path as the sample iUumination Ught, so that IR micro-spectroscopy can be performed on the sample at the center of the viewing field. Because of their design, they are also equipped with a number of convenient methods for enhanced sample visualization. These include polarized light (visible and IR), fluorescence illumination and differential interference contrast (DIG), all of which are well known and frequently used to identify biological sample histology. 

Confocal scanning optical microscopy Differential interference contrast Energy dispersive x-ray spectroscopy... 

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