Diethyl pyrocarbonate, effect

Characterization of the modified plastocyanins was by Inductively Coupled Plasma Emission Spectroscopy to analyze for Ru and Cu (1 1 ratio), and by HNMR spectroscopy. In the HNMR characterization the C2H resonance of His59 at 8.2 ppm is seen to be lost due to paramagnetic line broadening effect of the attached Ru(III), Fig. 13 [50]. In a further test it is known that the His59 s of both native plastocyanins react with diethyl pyrocarbonate (DEPC) to give an JV-ethoxyhistidine derivative, (12), which absorbs strongly at 238 nm (e 2750M- cm-i), Fig. 14 [133]. 

The attachment of C-ascorbic acid (100 fxM) to 5 mg/ml bovine serum albumin was asessed after 2-hr incubation at pH 7.4 and 37° C. The effect of the metal chelator, 1 mM diethylenetriamine pentaacetic acid, 25 mM glutathione, 25 mM oxidized glutathione, and 1 mM diethyl pyrocarbonate, DPC, is shown. Reprinted with permission (Hunt and Wolff, 1991b). 

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