Diethofencarb-resistant mutants

To elucidate the mechanism of negatively correlated cross resistance and mode of action of diethofencarb, we isolated diethofencarb resistant mutants from mutagenized F914 strain. 

In another cross of moderately diethofencarb-resistant mutants (strain F421, F423 and F513) to the wild type strain, we obtained... 

However difference aspect was observed in resistance to MBC between N. crassa isolates in laboratory and B. cinerea isolates from the field. Laboratory-generated mutants of N. crassa showed various levels of resistance to MBC, and a specific one showed negatively correlated cross resistance to diethofencarb. Others showed double resistance to these chemicals. On the other hand, most of the benzimidazole resistants isolated from the field, especially in in the case of B. cinerea, were supersensitive to diethofencarb. This difference between laboratory and field strains may derive from a difference in fitness among the resistant strains of the plant pathogen. 

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