Diels-Alderases discoveries

The Diels-Alder reaction, probably the most widely used methodology in organic synthesis today, has contributed greatly to the development of mechanistic and theoretical chemistry. The recent discovery of a Diels-Alderase enzyme has provided insights into the mechanism of biosynthetic cycloaddition. 

In this article, we described the outline attaining to discoveries of the Diels-Alderases which enzymatically catalize the Diels-Alder reaction. At present, it is certain that three Diels-Alderases, solanapyrone... 

Finally, a potential breakthrough in biocatalysis that will require strong synergy between chemistry, molecular biology, and bioinformatics in order to render it suitable for industrial apphcations is the de novo creation of proteins. This nascent field promises exciting discoveries, as novel biocatalysts could be designed to catalyze reactions where biochemical counterparts are missing (e.g., Kemp elim-inase, intermolecular Diels-Alderase) [152-159]. 

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