Diastereometric complexes

Chiral separation in capillary electrophoresis is usually achieved by the addition of chiral complexing agents to form in situ diastereometric complexes between the enantiomers and the chiral complexing... 

Chiral separation in CE is usually achieved by the addition of chiral complexing agents to form in situ diastereometric complexes between the enantiomers and the chiral complexing agent. Many of the chiral selectors successfully used in HPLC can also be applied in CE, and thus the experience from both HPLC and CE can be transferred to CEC. Dtrring the last few years, interest in CEC has increased due to the improvement in the preparation of capillary columns and in the stability and efficiency of separations.A limited but dramatically increasing number of chiral separations in CEC have been... 

In HPLC, the direct serial coupling of achiral and chiral columns for the simultaneous achiral/chiral separation of a range of components in a complex mixture was not feasible due to either the increased backpressure or the different mobile-phase requirement. However, this concept is not restricted for SFC because the supercritical fluid not only has a significantly lower viscosity than a liquid but is also an effective eluent for both chiral and achiral stationary phases [52], As an example, an SFC-MS approach using the coupled achiral/chiral column combination was reported to significantly enhance the separation efficiency for the reaction mixtures of cinna-monitrile and hydrocinnamonitrile intermediates to determine the diastereometric/ enantiometric composition of the final product in one step [52], Characterization of... 

Scheme 25. Diastereometric 7t-complexes during the AHR of 84 in the presence of (R)-BINAP...

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