Diastase Activity

The diastase activity was traditionally determined according to the Schade method in the earlier years (Schade et al., 1958). One unit of diastase activity (or more specifically, a-amylase), DN, is defined as that amoimt of enz)nne that converts 0.01 g of starch to the prescribed endpoint in 1 h at 37 °C under the experimental conditions. In this assay, a standard solution of starch, which reacts with iodine to produce a color solution, is used as a substrate for honey enzymes under the standard conditions (Rendleman, 2003). A recently developed procedure uses an insoluble, dyed starch substrate (Persano Oddo and Pulcini, 1999). As this substrate is hydrolyzed by ot-amylase, soluble dyed starch fragments are released into solution. After reaction termination and insoluble substrate removal by centrifugation, absorbance of the supernatant solution (at 620 nm) is measured. The absorbance is proportional to the diastase activity. This procedure has been widely adopted in the honey industry due to the convenience of a commercially available substrate and the simple assay format. 

Enzyme activity can indicate the exposure of honey to heating and long storage. This criterion is not more accurate than the HMF content value because enzyme activities vary with honey samples. The diastase activity is usually associated with heat treatment. However, its activity gives only an indication about the processing (heat treatment) of the honey but is not suitable for the detection of the origin. 

Serra Bonvehi, J. (1988). Determination of methyl anthranilate in citrus honey (Citrus sp.) of eastern Spain and its influence on the diastase activity of the honey. Alimentaria 197,... 

Effects of Thermal Treatment and Storage on Hydroxymethylfurfural (HMF) Content and Diastase Activity of Honeys Collected from Middle Anatolia in Turkey... 

This study has two goals The first one is to investigate the effects of storage on HMF formation and diastase activity of honey. Second one is to determine HMF level and formation kinetics of honey after heating process. For this purpose 40 samples of honey were collected from Middle Anatolia and surrounding areas. The physicochemical properties of honey collected were determined. The obtained data were compared with results of other researchers. 

Keywords Anatolia, diastase activity, honey, hydroxymethylfurfural... 

Thermal treatment, applied to honey, may destroy vitamins and bionutrients, and produce a simultaneous decrease in diastase activity and an increase in HMF content. Honey treatment temperature and time must be limited when pasteurising and stabilising it both diastase activity and HMF content are national and international parameters used as controls so as to limit thermal treatment application. HMF can be formed by hexose dehydration in acid media or by the Maillaid reaction [11,12]. According to Ibarz et al., HMF formation can be described by a second order kinetics (auto-catalytic), with the following equation as expression model [13] ... 

Calculation One unit of diastase activity, expressed as degrees diastatic power (DP°), is defined as that amount of enzyme contained in 0.1 mL of a 5% solution of the sample enzyme preparation that will produce sufficient reducing sugars to reduce 5 mL of Fehling s solution when the sample is incubated with 100 mL of the substrate for 1 h at 20°. 

Calculate the diastase activity, expressed as DP°, of the sample by the formulas... 

Diaminohexanoic Acid Hydrochloride, 229 Diammonium Phosphate, 27, (S3)4 Diastase Activity (Diastatic Power), 793 Diatomaceous Earth, 120, (S2)8 Diatomaceous Silica, 120, (S2)8 Diatomite, 120, (S2)8... 

Catalase Activity, 791 Cellulose Activity, 791 Chymotrypsin Activity, 793 Diastase Activity (Diastic Power), 793 a-Galactosidase Activity, 794 [i-Glucanasc Activity, 795 Glucoamylase Activity... 

Tosi E., Martinet R., Ortega M., Lucero H. Re E. (2008). Honey diastase activity modified by heating. Food Chemistry Vol. 106, pp. 883-887. 

The most prominent enzymes in honey are a-glucosidase(invertase or saccharase), a- and P-amylases (diastase), glucose oxidase, catalase and acid phosphatase. Average enzyme activities are presented in Table 19.14. Invertase and diastase activities, together with the hydroxymethyl furfural content, are of significance for assessing whether or not the honey was heated. 

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