Deuteration random fractional

Although the first NMR spectra of deuterated proteins were published already in 1968, the deuteration was mainly employed for the purpose of simplifying one-dimensional proton spectra.53,54 Later in the 1980s, LeMaster and co-workers employed random fractional deuteration for the assignment of thioredoxin using homonuclear two-dimensional spectroscopy.55 However, it was realized that deuteration significantly improves the spectral quality of homonuclear multidimensional NMR experiments. 

LeMaster, D. M. and Richards, F. M. (1988). NMR sequential assignments of E. coli thiore-doxin utilizing random fractional deuteration. Biochemistry, 27, 142-150. 

Figure 13 illustrates the comparison between the experimental xsans(F) data for a pair of systems that are labeled by Graessley et al. [28] as H38/D25 and H25/D38 and the BLCT xsansCF) as calculated from Eq. 37 for the set of caa. sbej ab> and y specified above. The notation H38/D25, for example, denotes the isotopic blend of hydrogenated (H) and deuterated (D) polybutadiene random copolymers, and the numbers indicate the fractions of 1,2 units in each of the blend components. The experimental points [28] are designated in Fig. 13 by circles and triangles, whereas the fines represent the theoretical fits. Figure 13 demonstrates that the theory reproduces the overall values of the X parameters within the experimental error bars of 1.5 x 10 [28]. 

Figure 1. Scattered intensity from a statistical gel (a semi-dilute solution of polymer volume fraction ([) = 0.1, transformed into a "gel" by random reaction of tie-points between the chains concentration of crosslinking molecules Cx=0.8%, in moles per mole of "monomer unit"). The neutron scattering contrast between the network and the solvent is achieved by swelling the gels with deuterated toluene. The signal from the gel is compared to that of a semi-dilute solution of the same polymer concentration, here for two swelling degrees only left, nearly that of preparation right maximum swelling approximately...

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