Organic acids determination

Many methods have been used to determine organic acids in foods, including volumetric, electrochemical, enzymatic, and chromatographic (paper, thin-layer, gas-liquid, or HPLC) methods. However, most of these are not able to assay organic acids comprehensively for example, the enzymatic methods are specific kits for individual organic acids (i.e., they only detect one of the acids present). Therefore, to analyze the sample comprehensively using enzymatic methods would be extremely time consuming and costly, as the analysis would have to be run separately for each acid and would require several kits. 

Langbehn et al. [117] used supercritical fluid chromatography to determine organic acids and ketones in soil. 

Mato, I., Suarez-Luque, S., and Huidobro, J.F. 2005. A review of the analytical methods to determine organic acids in grape juices and wines. Food Research International 38 1175-1188. 

Caccamo F, Carfagnini G, Di Corcia A, Samperi R (1986) Improved high-performance liquid chromatographic assay for determining organic acids in wines. J Chromatogr 362 47-53... 

Chalmers, R.A., Watts, R.W.E. and Lawson, A.M. (1977b), A comprehensive screening method for determining organic acids in the diagnosis of metabolic diseases in acutely sick infants and children. Ann. Clin. Biochem., 14,149. 

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