Determination of kLa in Bubble-free Bioreactors

In the absence of viable cells in the bioreactor, an effective mass transfer coefficient can be obtained from 

C Q2 is the dissolved oxygen concentration in equilibrium with the oxygen in the air supply at the operating pressure and temperature of the system. Since the dissolved oxygen concentration is measured as percent of saturation. DO (%), the above equation can be rewritten as 

The constants DO 0o% and DO0% are the DO values measured by the probe at 100% or 0% saturation respectively. With this formulation one can readily analyze the operation with oxygen enriched air. 

Using the data shown in Table 17.12 you are asked to determine the effective kLa of the bioreactor employing different assumptions  

Finally, based on your findings discuss the reliability of each solution approach. 

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