Determination by fluorescence quenching

Eftink, M.R. and Chiron, C.A. (1976) Exposure of tryptophanyl residues in proteins quantitative determination by fluorescence quenching studies. Biochemistry 15, 672-679. 

Determined by fluorescence quenching Determined by UV absorption values. 

The ket values have been determined by fluorescence quenching of RAcrH+ by electron donors [79]. Figure 5 exhibits the dependence of the observed rate constant for intermolecular electron transfer (ket) on AGet [Eq. (6)], which is assumed to be the same as AGet (Scheme 7) in MeCN (part a) and in... 

The significance of the surfactant micelle-polymer hydrophobe stoichiometry is nicely illustrated if the viscosity is presented as a function of the concentration of micelles (or hydrophobic microdomains), as determined by fluorescence quenching. In Figure 20.13 viscosity... 

Figure 20.13. The viscosity of a solution of a hydrophobically modified water-soluble polymer and a surfactant compared to the concentration of micelles (determined by fluorescence quenching). The viscosity (filled circles) has a maximum when the micelle concentration starts to increase. The system illustrated is that of hydroxyethyl cellulose (1 wt%) and sodium dodecyl sulfate (SDS). (S. Nilsson, Ph. D. Thesis, Lund University, Sweden, 1999)...

Values determined by fluorescence quenching with the exception noted below. Data are from Haber and Richards (13). 

Peuravuori, J., Partition coefficients of pyrene to lake aquatic humic matter determined by fluorescence quenching and solubility enhancement. Anal. Chim. Acta, 429, 65, 2001. 

H5.1 showed an emission at 330 nm following excitation at 280 nm. The emission derived from the hydroquinone units of H5.1. Addition of the viologen G5.1 induced fluorescence quenching. The association constant of viologen G5.1 with H5.1, determined by fluorescence quenching, was 10 times higher than that of pyridinium G5.2 with H5.1 (Table 5.1, runs 1... 

TLC was used for both qualitative (26-28) and quantitative analysis (29-31) of flavins in different matrices. The separation and quantitative determination by fluorescence quenching of the B-complex vitamins in some pharmaceutical products was performed using a newly synthesised carbamide formaldehyde polymer aminoplast with solvent systems 1-butanol/water/acetone (25 9 5, v/v) and 1-butanol/methanol/benzene/water (20 10 10 8, v/v) (29). 

The highest reported binding constant to date for a mono-macrocyclic calixarene or calixarene analogue receptor and Cgo is 3.48 x 10 which was ascribed to the Ag(I) A-heterocyclic carbene-bridged calixarene 48 synthesized by Quin et al. [72]. This binding constant for the 1 1 complexation was determined by fluorescence quenching titration study of in acetonitrile at 298 K (Fig. 33.33). 

After separation, the enantiomers were eluted on the above-layer portion by using the continuous development method and were determined by fluorescence quenching at 254 nm, allowing to perform in situ quantitative measurements by using a Shimadzu CS9000 scanning densitometer. 

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