Detection of sulphite-reducing, spore-forming anaerobes

Detection of sulphite-reducing, spore-forming anaerobes Liquid enrichment 

20 to 50 ml of water is mixed with the same quantity of a double-strength 

The culture is incubated for up to 44 hours at 37 1 °C. A positive reaction is indicated by the liquid culture medium turning black. 

Approx. 3 g pyrogallol is thoroughly moistened with 8 to 10 ml of a 10 % solution of sodium carbonate (Na2C03) in the lower part of a Petri dish. The dish with the membrane-filter culture to be analyzed is immediately placed on top and the two dish halves are sealed to one another in an airtight manner, Pyrogallol binds the oxygen present in the system. Care must be taken that the culture medium layer with the membrane filter is not wetted by the liquid in the lower dish part as a result of careless handling. 

The cultures are incubated at 37 ° 1 °C and evaluated after not longer than 44 4 hours. Black colonies, which can if necessary be counted and related to the quantity of water used, are positive. 

---

See also in sourсe #XX -- [ ]

---