Detection method, DIGE

At present, there are advanced difference gel electrophoresis (DOGE) Systems and 2-D fluorescence difference gel electrophoresis (2-D DIGE) which enable the analyst to use simultaneously modern (more precise) methods of fluorescent analysis with 2-D electrophoresis (using internal patterns), aided by a fully integrated bioinformatics system. Such systems allow more complete differential protein analysis, while the application of internal standards eliminates differentiation between the intervals, thus ensuring that even the smallest differences will be detected irrespective of the multitude of components. This guarantees reproducibility of results and their statistical reliability. Such assays are one of the platforms employed in the research based on the proteomics method. 

These advantages make the ion-y reactions a particularly useful elemental analysis technique. Because the detection technique is different from the detection of both X-rays in PIXE and particles in RBS and NRA, the method got in practice a distinctive name particle-induced 7-ray emission (PIGE), where the word particle means mostly proton. PIGE is often used simultaneously with PIXE, and its suitability for the analysis of light elements (mainly Li, B, F, Na, Mg, Al, and Si) complements the performance of PIXE well. Among y-ray emissions induced by various types of particles, the deuteron-induced 7-ray emission (DIGE) deserves the most practical interest, because it is also sensitive to C, N, and O, and its high cross sections... 

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