Designed model proteins

Properties Once Considered Unique and Essential to Life Are Unexpectedly Found in Designed Model Proteins... 

Figure 1.3. A Hill plot of the set of designed elastic-contractile model proteins shown in Figure 1.2 with Hill coefficients, n, ranging from 1.5 to 8.0. B Hill plot of myoglobin (n = 1) and hemoglobin (n = 2.8). It is shown that the vaunted hemoglobin positive cooperativity is relatively small compared with that of designed elastic protein-based polymers and, in particular, of designed Model protein v.

Phenomena exhibited during protein function and dysfunction (disease) parallel those phenomena exhibited during function of designed model proteins. Examples of this coherence of phenomena follow in a cursory introductory form. Detailed considerations are given in Chapters 7 and 8 based on the physical mechanisms developed in Chapter 5. 

These results of 18 years ago, demonstrating the capacity of de wovo-designed model protein-based machines for the conversion of chemical energy into mechanical work, remain unex-... 

These experimental demonstrations of our capacity to design model proteins that perform key biological energy conversions constitute our access to the question o/What Sustains Life. 

When a properly designed model protein contains both protonation/deprotonation chemical... 

Another example is the Principle of Le ChStelier, which may be stated as follows For any system at rest (at equilibrium) the introduction of a stress (in our case an input energy) causes the system to react in such a way as to relieve the stress (in our case by an output energy). This principle reasonably describes protein-catalyzed energy conversion, that is, the function of protein-based machines. Under prescribed conditions, properly designed model protein-based machines exhibit a behavior where for each action there is a reaction. In section 5.4, regardless of the action, which was any one of several different input energies, the performance of mechanical work was the reac-... 

The AGap-driven interconversion between states of solubility and insolubility exhibits positive cooperativity and as such becomes the physical basis for the allosteric effect of Monod. Thus, AG.p, demonstrated with designed model proteins, results in a positively cooperative interchange between hydrophobic... 

In Chapter 5, based on an inverse temperature transition due to hydrophobic association in water, a set of Axioms were derived from the phenomenological demonstration that de novo designed model proteins could efficiently interconvert the set of energies interconverted by living organisms. Then there followed a series of experimental results and analyses that defined the comprehensive hydrophobic effect. 

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