Polyacrylamides derivatized

Cuatrecasas, P. (1970). Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J. Biol. Chem. 245, 3059-3065. 

Inman, J.K., and Dintzis, H.M. (1969) The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents. Biochemistry 8, 4074-4082. 

Brandley and Schnaar [140] immobilized a synthetic nonapeptide, Tyr- Ala-Val Thr-Gly Arg-Gly-Asp-Ser, on a polyacrylamide gel, which had been prepared by a ternary copolymerization of acrylamide, bisacrylamide and the acrylic ester of. V-hydroxysuccinimide. They reported that Balb/c 3T3 mouse fibroblast cells (in Hepes-buffered Dulbecco s modified Eagle medium) adhered readily to the peptide-derivatized surfaces, even in the absence of serum, although long-term cell growth required the presence of serum. It was noticed that reference nonapeptide. Tyr-Arg-Leu-Glu-Asp-Pro-Ala-Met-Trp, which has no RGD sequence, failed to promote cell-attachment. 

CZE separation of metal ions (Zn, Cd, Al), which were ElQS-derivatized, was achieved in a microchip. To enhance separation resolution without using an excessively long channel or high voltage, EOF was eliminated by coating the channel wall with polyacrylamide, and separation was carried out in the cathodic mode. The use of a fused quartz substrate resulted in a reduction in fluorescent background, leading to a LOD of 30-57 ppb [352]. 

Chemical derivatization of the capillary wall has also been used to alter the properties of the interface. Derivatization using trichlorovinylsilane followed by copolymerization with polyacrylamide is one approach. Another effective reagent is (o -methacryloxypropyl)trimethoxysilane, followed by cross-linking the surface-bound methacryl groups with polyacrylamide. 

Fig. 6.10. Isocratic electrochromatography of an oligosaccharide ladder in a capillary filled with a macroporous polyacrylamide/poly(ethylene glycol) matrix, derivatized with C4 ligands (15%) and containing vinylsulfonic acid (10%) (Reprinted with permission from [35]. Copyright 1997 American Chemical Society). Conditions capillary length, 50 cm (40 cm effective length) x 100 pm i.d. mobile phase, 0.1% aqueous acetic acid containing 5% (v/v) acetonitrile field strength, 600 V/cm, injection, 100 V/cm for 5 s sample concentration, 30 mg/mL in derivatization solvent and thereafter diluted 1 100 in the mobile phase.

Fig. 1 Isocratic electrochromatography of peptides in a capillary filled with a macroporous polyacrylamide-polyethylene glycol matrix, derivatized with a C12 ligand (29%) and containing acrylic acid. Conditions mobile phase, 47% acetonitrile in a buffer voltage, 22.5kV (900 V/cm), 7 [jim sample concentration, 4-10 mg/mL detection, UV absorbance at 270 nm other conditions are described in Ref. 5. (From Ref. 5 reproduced with permission of the authors and the American Chemical Society.)...

Isoelectric focusing is a steady-state technique, and its application in capillaries requires the partial or total supression of electroosmotic flow. Capillaries used for CIEF can be derivatized with methylcellulose or linear polyacrylamide, to block the surface silanol groups, whose ionization is responsible for electroosmotic... 

Capillary isoelectric focusing was performed in a 75-pm i. d. capillary where the surface silanols had been derivatized with linear polyacrylamide. A mixture of proteins having pi values between 6 and 8 were focused using ampholytes covering the pH 3-10 range, using an anolyte of 20 mAf HC1 and a catholyte of 20 mM NaOH. A static separation pattern was indicated by a current minimum that occurred after 90 min at 15 kV. How can salt mobilization be used to elute the separation pattern at the anodic end of the capillary ... 

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