Derivatization organic trace analysis

We inferred that these properties might be exploited in a series of unique derivatizing reagents designed specifically for trace analysis of organic compounds using HPLC separation and fluorescence detection. The use of these pyridones for the analytical purposes reported here is based on their acidic properties. Treatment of a lH-2-pyridone with a base converts the pyridone to its salt. 

Several derivatization techniques have been developed for trace analysis of bromate in drinking water (see Section 10.1), because concentrations below 2 pg/L cannot be determined by suppressed conductivity detection without preconcentration [55]. Even at lowest microgram/Liter levels, bromate has been classified as carcinogenic by the World Health Organization (WHO) and the American Environmental Protection Agency (EPA) [56]. Lower detection limits cannot be achieved by enlarging the injection volume, because sample matrix... 

Insofar as the derivatization can be considered one of the stages of sample preparation for chromatographic analysis, it can be combined with other procedures, for instance, the preconcentration of traces of analytes. For example, the yield of solid-phase extraction or microextraction of organic compounds from aqueous solutions with modified silica gels is better for more hydrophobic substances the preliminary conversion of acidic compounds into suitable derivatives is recommended. ... 

An interesting example of trace metal GC-MIP analysis, utilizing derivatization for organic functional group determination, involves the reaction of ferroceneboronic acid with diols. Brooks and Cole showed quantitative derivatization to give products with good GC behavior... 

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