Derivatives as Surface Modification Agents

React for at least 1 hour at room temperature with mixing. Some reactions have been done for up to 24 hours at elevated temperatures (e.g., 40°C). 

Purify the DTPA-dendrimer using dialysis, size exclusion chromatography, or spin-tube concentrators having a molecular weight cutoff of 5,000 Daltons. 

Other chelate-dendrimer constructs may be prepared using alternative bifunctional chelating agents according to Chapter 10. 

Dendrimer-PEG3-Ligand coupled through triazole linkage 

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