Depository Smart Compound

The master solutions in the liquid master store are also used to produce multiple copies of single-use aliquots in 384-microtube plates, which are used for two processes (i) loading of the Smart Compound Depository with new compound aliquots, and (ii) replenishment of frequently ordered compounds in this depository. The continuous replenishment of frequently ordered compounds through the Liquid Master Store... 

Replenishment of frequently ordered compounds in the Smart Compound Depository... 

Table 2 Sample aliquoting tree for the supply of the vast amount of samples needed for high-throughput screening. The Liquid Master Store assembles editions of master solutions for preparation of the compound aliquots needed for multiple HTS projects. The Smart Compound Depository delivers the compound subsets from the HTS library needed for hit validation and secondary screening...

The plate-to-plate tube transfer at -20 °C in the Liquid Master Store and in the Smart Compound Depository are similar robotic operations that can be combined in one automated sample bank. The combination of 96-tube and 384-microtube plate-to-plate tube transfer in one system might be the preferred solution for sample management of small- and medium-sized HTS libraries. 

As soon as the tube count per compound drops below a defined threshold (e.g. 2 tubes) for a minimum of 384 compounds in the Smart Compound Depository, 384 of these compounds are ordered from the Liquid Master Store in order to prepare new aliquots for replenishment of samples. 

---