Deoxyribose degradation

Perjesi P, Kuzmaa M, Fodora K and Rozmera, Z. 2007. Application of crocin bleaching and deoxyribose degradation tests to assess antioxidant capacity of capsaicinoids and some selected flavonoids. Eur J Pharm Sci 32 39. 

Deoxyribose reacts with hydroxyl radical with a rate constant of 3.1 x 109 M 1s 1. Deoxyribose degraded by hydroxyl radical produces malonyldialdehyde which reacts with thiobarbituric acid (TBA) to produce a pink chromophore absorbing at 532 nm (Halliwell and Gutteridge, 1981). 

The system being assessed should not interfere with the measurement of the deoxyribose degradation products. This can be checked by showing that it does not inhibit when added to the reaction mixture at the end of the incubation with the thiobarbituric... 

Hydroxyl Radicals in Biochemical Systems Deoxyribose Degradation... 

Halliwell, B. Grootveld, M. Gutteridge, J., M. Methods for the measurement of hydroxyl radicals in biomedical systems deoxyribose degradation and aromatic hydroxylation. Methods Biochem. Anal. 33 59-90 1988. 

The effects of berbamine, oxyacanthine, and berberine on 5-lipoxygenase lipid peroxidation in phospholipid liposomes induced by 2,2 -azo-(bis-2-amidinopropane)(AAPH), deoxyribose degradation, and their reactivities against the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) were studied. None of the alkaloids showed any appreciable effects in the inhibition of 5-lipoxygenase. Reactivity against DPPH increased in the following order berberine < oxyacanthine < berbamine. Pro-oxidant effects of the alkaloids were excluded, since deoxyribose degradation was not influenced as determined by the release of malondialdehyde [200],... 

The scavenging and iron-reducing properties of a series of benzylisoquinoline alkaloids and synthetic benzylisoquinoline compounds was studied. Glaucine was found to act as a scavenger of the hydroxyl radical in the deoxyribose degradation by Fe+3-EDTA + H202 [265],... 

Gutteridge, J.M.C., Quinlan, G.J., and Wilkins, S., Mitomycin C-induced deoxyribose degradation inhibited by superoxide dismutase a reaction involving iron, hydroxyl and semiquinone radicals, Lett, 167,37, 1984. 

Effect of Phytic Acid Hydrolysis Products on Deoxyribose Degradation Assay... 

Figure 3. Effect of phytic acid hydrolysis products on iron ion4nduced deoxyribose degradation.

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