Deoxyribose assay

Aruoma, O. 1. (1994). Deoxyribose assay for detecting hydroxyl radicals. Methods in Enzymology, Vol. 233, pp. 57-66, ISBN 978-0-12-182148-7. 

Gutteridge, J.M. Halliwell, B. (1988). The deoxyribose assay an assay both for free hydroxyl radical and for site-specific hydroxyl radical production. Biochemical Journal, Vol. 253, (April 1988), pp. 932-933, ISSN 0264-6021. 

Aruoma Ol (1994) Deoxyribose assay for detecting hydroxyl radicals. Methods Enzymol 233 57-66 Ashton L, Buxton GV, Stuart CR (1995) Temperature dependence of the rate of reaction of OFI with some aromatic compounds in aqueous solution. J Chem Soc Faraday Trans 91 1631-1633 Asmus K-D, Mockel H, Flenglein A (1973) Pulse radiolytic study of the site of OFI radical attack on aliphatic alcohols in aqueous solution. J Phys Chem 77 1218-1221... 

Deoxyribose assay for the detection of hydroxyl radical production in cellular systems... 

This simple reaction has been exploited extensively in several cell-free systems for studying oxygen-radical reactions. The deoxyribose assay is a sensitive assay for hydroxyl radicals. However, it may not be specific for this species, and it has been suggested that deoxyribose can be degraded by ferryl haem species, although we have found no such interaction in our conditions. 

The deoxyribose assay is a simple test tube assay to determine the antioxidant or pro-oxidant properties of test compounds against carbohydrates [151-152]. In the deoxyribose assay, OH are produced from the reaction of H2O2 with Fe2+ (Fenton reaction), where the latter is formed by the ability of ascorbic acid to reduce ferric iron (Fe3+) to ferrous iron (Fe2+) (Eq. 27). 

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