Deoxyribonuclease activity determination

N. B. Kmrnick. The determination of deoxyribonuclease activity by methyl green application to serum. Arch. Blochem. 29 41-53 (1950). 

R. Osman, PL. Kan, G. Awad, N. Mortada, A.E. El-Shamy, O. Alpar. Enhanced properties of discrete pulmonary deoxyribonuclease I (DNasel) loaded PLGA nanoparticles during encapsulation and activity determination. Int J Pharm 408, 257-265,2011. 

The TNF-a-induced DNA fragmentation may have been caused by caspase-activated deoxyribonuclease (CAD) in PC-12 cells. In non-apoptotic cells, CAD is present as an inactive complex with the inhibitor jOvd p3,24]. During apoptosis, caspase-3 inactivates ICAD, leaving CAD free to function as a nuclease [25]. Therefore, we used die fluorogenic substrate, Ac-DEVD-MCA, to determine whether the caspase-3 in PC-12 cells was activated by treatment with TNF-a and/or crocin. As shown in... 

The role of the divalent metal ions present in natural phosphodiesterases became clear in bovine pancreatic deoxyribonuclease I (DNase I), the first endonuclease structure determined by X-ray crystallography. The nucleophilic attack of a water molecule activated by a histidine residue is facilitated by the interaction of a calcium ion with the phosphate group to be cleaved (291). Glutamic and aspartic residues involved in magnesium binding have been identified in the crystal structure of four type II restriction enzymes EcoRl (292), EcoRV (293), Pvull (294), and BamHl (295), as well as in that of the repair... 

---